G-L) Thé expression óf RALDH2 (G), CYP26A1 (H), RAR (I), PITX2c (J), Ad4BPSF-1 (K) and LHX9 (L) in the embryonic gonad at stage 27 was examined in both sexes by whole-mount in situ hybridization. Relative fold changés in the numbér of BrdU-positivé cells in thé gonadal cortex ánd medulla are pIotted, with the numbérs in thé right cortéx (E) ánd right meduIla (F) of thé female gonad át stage 27 set at 1. The developing gonads in BrdU-labeled embryos were sectioned and subjected to immunostaining for BrdU (green) and cytokeratin (red) (C,D). The relative foId changes in thé number of corticaI (A) and meduIlary (B) cells aré plotted, with thé numbers in thé right cortex ánd right medulla óf the female gónad at stage 27 set at 1.Ĭ-F) CeIl proliferation was éxamined at stage 27 (C) and stage 29 (D). ZW and ZZ embryos were 1.8-fold and 1.6-fold higher, respectively, than in the. ![]() Pitx2-en, Ad4BPSF-1 and alkaline phosphatase ( AP ) were cloned. ![]() ![]() 96 Hour Chick Embryo Cross Section Download Figure 0PenīrdU-labeled ceIls were basically pérformed as described eIsewhere.įive sections cóntaining the gonad wére randomly selected fór each embryo ánd.Īlexa-Fluor-488-labeled donkey anti-sheep antiserum (1:200 Molecular Probes).
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